Journal: Allergy

Article Title: Vaccine‐Induced Anti‐ IgE Antibodies Neutralize Free IgE but Fail to Bind and Activate Mast Cell ‐Displayed IgE

doi: 10.1111/all.16530

Figure Lengend Snippet: Purified IgGs from CuMV TT ‐Cε3‐Cε4 recognize IgE‐mannose. Purified IgG was characterized by ELISA. (A) Shown is the ELISA setup to detect mannose‐specific IgG (left panel) and mean ± SEM OD450 values (right panel). (B) Shown is the ELISA setup to block IgG binding using mannose‐binding Galanthus Nivalis Lectin (GNL) and mean ± SEM OD450 values. (C) Shown is the ELISA setup to block IgG binding using WT IgE or mannose‐deficient IgE (F1) to block IgG binding and mean ± SEM OD450 values.

Article Snippet: Moreover, the mannose binding lectin Galanthus Nivalis lectin (GNL) blocked IgG binding to wild‐type IgE (Figure ).

Techniques: Purification, Enzyme-linked Immunosorbent Assay, Blocking Assay, Binding Assay

Journal: Oxidative medicine and cellular longevity

Article Title: Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo .

doi: 10.1155/2022/5696686

Figure Lengend Snippet: Figure 6: Hematoxylin and eosin staining in kidney samples. Tubular injury, glomerulosclerosis score, and Masson staining after treatment of mice with CS. (a) C57BL/6 mice were treated with CSE and harvested for 4 months. H&E staining was examined in kidney samples. The cell nuclei were stained blue by hematoxylin. Both the extracellular matrix and cytoplasm were stained by eosin (pink). Scale bar: 50 μm. (b) Tubular injury and glomerulosclerosis were analyzed in kidneys (N = 5). The data are presented as the means ± SD. Twenty fields of view per kidney. ∗p < 0:05 and ∗∗∗p < 0:001 compared to the normal group samples. (c) Kidneys were stained with Masson’s trichrome. Scale bar = 50 μm. Immunohistochemistry for senescence-associated β-galactosidase, autophagy-related proteins, glucose-6-phosphate dehydrogenase, and kidney samples of after treatment of mice with CSE. (d) C57BL/6 mice were treated with CSE and then harvested after 4 months. IHC staining of SAβgal was examined in kidney samples. (e) IHC staining of LC3 was examined in kidney samples. (f) IHC staining of G6PD was examined in kidney samples. Scale bar: 50 μm.

Article Snippet: UltraVision protein block buffer was applied to analyze the kidney after treatment with G6PD (Proteintech), LC3 (MBL) or β-gal (Invitrogen) antibody in 3% BSA overnight at 4°C.

Techniques: Staining, Immunohistochemistry

Journal: Oxidative medicine and cellular longevity

Article Title: Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo .

doi: 10.1155/2022/5696686

Figure Lengend Snippet: Figure 7: Schematic of the putative mechanism illustrating the CSE-induced G6PD, autophagy-related, fibrosis-related, and senescence- related protein expressions in kidney cells. Kidney cells were induced by CSE via several related pathways that increase the expression of the fibrosis-related proteins, autophagy-related proteins, and senescence-related proteins in kidney cells. CSE regulates senescence and fibrosis via inhibition of Hsp27, G6PD, Klotho, and autophagy.

Article Snippet: UltraVision protein block buffer was applied to analyze the kidney after treatment with G6PD (Proteintech), LC3 (MBL) or β-gal (Invitrogen) antibody in 3% BSA overnight at 4°C.

Techniques: Expressing, Inhibition